Calibration of 3-hydroxybutyrate assays.
نویسندگان
چکیده
منابع مشابه
An Electrochemical Enzyme Biosensor for 3-Hydroxybutyrate Detection Using Screen-Printed Electrodes Modified by Reduced Graphene Oxide and Thionine
A biosensor for 3-hydroxybutyrate (3-HB) involving immobilization of the enzyme 3-hydroxybutyrate dehydrogenase onto a screen-printed carbon electrode modified with reduced graphene oxide (GO) and thionine (THI) is reported here. After addition of 3-hydroxybutyrate or the sample in the presence of NAD⁺ cofactor, the generated NADH could be detected amperometrically at 0.0 V vs. Ag pseudo refere...
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In this sensitive, reproducible method for determination of D-3-hydroxybutyrate (3-OHB) in plasma, it is converted to acetone by use of 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30)/lactate dehydrogenase (EC 1.1.1.27) coupled with acetoacetate decarboxylase (EC 4.1.1.4). The resulting acetone is detected by head-space gas chromatography. The lowest concentration of 3-OHB detectable in plasma wa...
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In this study we employed a capillary gas-liquid chromatographic-mass spectrometric (GLC-MS) method to measure the plasma concentrations of 3-hydroxybutyrate (3OHB) and 3-hydroxyisobutyrate (3-OHIB) in overnight fasted diabetic subjects and in normal subjects. Plasma contents of 3-hydroxybutyrate measured in this fashion were identical to those obtained by enzymatic assay using a commercial pre...
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We describe enzymic fluorometric methods of automated analysis for glucose, lactate, pyruvate, 3-hydroxybutyrate, glycerol, and alanine in perchloric acid extracts of blood. Unmodified Technicon AutoAnalyzer II apparatus is used. The usual concentrations of all these metabolites can be measured in as little as 0.1 ml of blood from a fasting subject. Within-batch and between-batch coefficients o...
متن کاملSimultaneous determination of D-glucose and 3-hydroxybutyrate by chemiluminescence detection with immobilized enzymes in a flow injection system.
A chemiluminometric flow-through sensor for the simultaneous determination of glucose (Glu) and 3-hydroxybutyrate (HB) in a single sample has been developed. Coimmobilized 3-hydroxybutyrate dehydrogenase/NADH oxidase/peroxidase, a support material, and coimmobilized glucose dehydrogenase/NADH oxidase/peroxidase were packed sequentially in a transparent PTFE tube. The tube was then placed in fro...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 31 1 شماره
صفحات -
تاریخ انتشار 1985